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甲狀腺球蛋白(TG)抗體產(chǎn)品應(yīng)用 WB, ICC, IHC-P, IHC-F, ELISA 規(guī)格 100ug
標(biāo)記物 未標(biāo)記
濃度 200ug/ml
克隆類型 多克隆 宿主 Rabbit,兔
Polyclonal Antibody to Thyroglobulin (TG)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. yb355Hu01
Clonality Polyclonal
甲狀腺球蛋白(TG)抗體Host Rabbit
Immunoglobulin Type IgG
Purification Affinity Chromatography
Applications WB, ICC, IHC-P, IHC-F, ELISA
Concentration 200ug/ml
UOM 100ug
Conjugate No Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant Thyroglobulin (Ser2083~Ala2333) expressed in E.coli.
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
甲狀腺球蛋白(TG)抗體MGSSHHHHHH SSGLVPRGSH MASMTGGM GRGS- SLTEKVSL DSWQSLALSS VVVDPSIRHF DVAHVSTAAT SNFSAVRDLC LSECSQHEAC LITTLQTQPG AVRCMFYADT QSCTHSLQGQ NCRLLLREEA THIYRKPGIS LLSYEASVPS VPISTHGRLL GRSQAIQVGT SWKQVDQFLG VPYAAPPLAE RRFQAPEPLN WTGSWDASKP RASCWQPGTR TSTSPGVSED CLYLNVFIPQ NVAPNASVLV FFHNTMDREE SEGWPAIDGS FLAAVGNLIV VTA
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against TG. It has been selected for its ability to recognize TG in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
甲狀腺球蛋白(TG)抗體Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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