產品編號 yb-1283R
英文名稱 Anti-ASPP2抗體
中文名稱 P53凋亡刺激蛋白2抗體
別    名 Apoptosis-stimulating of p53 protein 2; Bbp; Bcl2-binding protein; NY-REN-51 antigen; p53-binding protein 2; p53BP2; PPP1R13A; TP53BP2; Tumor suppressor p53-binding protein 2; apoptosis stimulating protein of P53 2; ASPP2_HUMAN.
 Anti-ASPP2抗體  
說 明 書 0.1ml  0.2ml  
研究領域 腫瘤  細胞生物  免疫學  細胞凋亡  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, 
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 124kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human ASPP2
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
產品介紹 background:
This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42. 

Subunit:
Binds to the central domain of TP53 as well as to BCL2. Interacts with protein phosphatase 1. Interacts with RELA NF-kappa-B subunit. This interaction probably prevents the activation of apoptosis, possibly by preventing its interaction with TP53. Interacts with APC2 and APPBP1. Interacts with DDX42 (via the C-terminus); the interaction is not inhibited by TP53BP2 ubiquitination and is independent of p53/TP53.

Subcellular Location:
Cytoplasm, perinuclear region. Nucleus. Note=Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42. 

Tissue Specificity:
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines. 

Similarity:
Belongs to the ASPP family. 
Contains 2 ANK repeats. 
Contains 1 SH3 domain.

 家族是近年來發現的一個新蛋白家族，它包括3個成員：ASPP1、ASPP2和iASPP。其中ASPP1和ASPP2能夠與p53蛋白結合增強該蛋白的促進細胞凋亡的功能，發揮抑制腫瘤的作用；而iASPP與ASPP1和ASPP2的功能截然相反，它能夠和ASPP1或ASPP2競爭性地與p53結合，抑制ASPP1或ASPP2的促進凋亡作用，具有癌基因的功能。三者的結構相似，但功能卻*不同，通過研究三者的關系、促進腫瘤細胞內ASPP1或ASPP2的高表達以及抑制iASPP的表達有可能促進腫瘤細胞的凋亡，成為*的新靶點.